Extraction and action of Exserohilum turcicum toxins in maize plants

Abstract

Spot blotch, caused by Exserohilum turcicum is important disease in maize, grass and sorghum. To demonstrate the action of the pathogen in plants the reaction is patogenicity important in procedures of improvement of plants. The form of substituting the patogenicity reaction, it is the action of toxins produced by the different isolates of the fungi. For this, isolates of E. turcicum from maize and grass were developed in Fries medium and than toxins were extracted. After ten days of the seed of maize germination, the leaves were collected and placed in Petriplates and receiving drops of maize and grass toxins, conidial suspension or water being later submitted to different analyses. The preliminary results demonstrated that maize plants with toxin of the isolate of maize fungi, presented necrosis besides, when the leaves were treated with conidial suspension they presented lesions. Like this, phytotoxins were capable to reproduce, the symptoms of the disease by the pathogens and that can be used in improvement plant processes.