Therapeutic ultrasound in the mitochondrial activity and differentiation of muscle cells C2C12

Authors

  • Beatriz Guimarães Ribeiro
  • Elis Cabral Victor
  • Vinicius Oliveira Cardoso
  • Paola Pelegrineli Artilheiro
  • Sandra Kalil Bussadori
  • Kristianne Porta Santos Fernandes
  • Raquel Agnelli Mesquita-Ferrari Universidade Nove de Julho - Uninove. São Paulo, SP

DOI:

https://doi.org/10.5585/conssaude.v13n3.4850

Keywords:

Cell differentiation, Creatine kinase, Muscle cells, Ultrasonic therapy.

Abstract

Introduction: The therapeutic ultrasound (US) has been widely used in clinical practice, but there are few studies on its effect on muscle regeneration. Objective: To evaluate the effects of the US on mitochondrial activity and differentiation of muscle cells C2C12 when applied concomitantly the induction of the differentiation process. Methods: Muscle cells were subjected to differentiation process by addition of DMEM culture medium supplemented with 2% horse serum and received concomitant treatment with US (pulsed at 20%, 3 MHz, 0.2 and 0.5 W/cm2, 5 minutes). The mitochondrial activity was assessed after 24, 48 and 96 hours by MTT assay and cell differentiation after one and three days for the activity of creatine kinase (CK). Results: There was no change in mitochondrial activity and CK in the groups receiving US treatment in different periods. Conclusion: In the evaluated parameters, the US was not able to change mitochondrial activity and differentiation of muscle cells C2C12.

Downloads

Download data is not yet available.

Author Biography

Raquel Agnelli Mesquita-Ferrari, Universidade Nove de Julho - Uninove. São Paulo, SP

Poutora e Mestre em Ciências Fisiológicas pela Universidade Federal de São Carlos, UFSCAR.

Published

2014-10-02

How to Cite

1.
Ribeiro BG, Victor EC, Cardoso VO, Artilheiro PP, Bussadori SK, Fernandes KPS, et al. Therapeutic ultrasound in the mitochondrial activity and differentiation of muscle cells C2C12. Cons. Saúde [Internet]. 2014 Oct. 2 [cited 2024 Jul. 8];13(3):429-35. Available from: https://periodicos.uninove.br/saude/article/view/4850

Issue

Section

Artigos