Interference of the pH, BSA and of phosphate buffer concentration on the response in the total protein determination by the biuret method

Abstract

In this work, the aim was to minimize the interference effect provoked by pH and the bovine serum albumin (BSA) and the phosphate buffer concentration on the total protein concentration determined by the biuret method, using a statistical model, established by response surface methodology (RSM). Results showed that the factors effects on protein concentration analysis were very significant and, in order to reduce these effects, it is necessary to use 0.01 M phosphate buffer at pH 7 and the protein concentration must be inferior, at 3 g/L.