In vivo killing of Staphylococcus aureus by toluidine blue-mediated photosensitization in an animal model wounds

Autores

  • Paulo de Tarso Camillo de Carvalho UNIDERP; Univ. Fed. Mato Grosso
  • Filipe Abdalla dos Reis UNIDERP
  • Ana Carulina Guimarães Belchior UNIDERP
  • Baldomero Antônio Kato da Silva UNIDERP
  • Daniel Martins Pereira UNIDERP
  • Nadia Cabral Bento UNIDERP
  • Ana Paula da Costa Marques UFMS
  • Geisa Poliane de Oliveira Uniderp, MS

DOI:

https://doi.org/10.5585/conssaude.v7i4.1415

Palavras-chave:

Photodynamic therapy, Toluidine blue, Wound infection.

Resumo

The aims of this study were to determine whether S. aureus could be killed by toluidine blue-mediated photosensitization in vivo in an animal model. Twelve Wistar rats divided into three groups (n = 12): Group I: Control Group, the wounds were made and submitted to the application of the laser without the drug photosensitizing; Group II: The implementation of wounds received the toluidine blue, without application of laser; Group III: it was used toluidine blue, and application of laser-Indio phosphide Gallium-Aluminum (InGaAIP)  660nm power and density of 20 Joules/cm2. Statistical analysis of CFU by analysis of variance Kruskal-Wallis test shows significant intraoperative difference, photodynamic therapy group (p < 0, 05), the Dunns post hoc test shows significant difference between Group I when compared to Group II treated with LLLT (p < 0001). The results of this study show that photodynamic therapy with toluidine blue has reduced the number of Staphylococcus aureus in vivo.

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Biografia do Autor

Paulo de Tarso Camillo de Carvalho, UNIDERP; Univ. Fed. Mato Grosso

Prof. Dr. do Deparmento de Fisioterapia da UNIDERP. Professor do Programa de Pós Graduação em Saúde e Desenvolvimento Regional da Universidade Federal do Mato Grosso do Sul- UFMS Postgraduate Program in Health and Development in West Central Region, Federal University of Mato Grosso do Sul, Campo Grande, Brazil. 2 Department of Physiotherapy, University for the Development of the State and Pantanal Region (UNIDERP), Campo Grande – MS

Filipe Abdalla dos Reis, UNIDERP

Department of Physiotherapy, University for the Development of the State and Pantanal Region (UNIDERP), Campo Grande – MS

Ana Carulina Guimarães Belchior, UNIDERP

Department of Physiotherapy, University for the Development of the State and Pantanal Region (UNIDERP), Campo Grande – MS

Baldomero Antônio Kato da Silva, UNIDERP

Department of Physiotherapy, University for the Development of the State and Pantanal Region (UNIDERP), Campo Grande – MS

Daniel Martins Pereira, UNIDERP

Department of Physiotherapy, University for the Development of the State and Pantanal Region (UNIDERP), Campo Grande – MS

Nadia Cabral Bento, UNIDERP

Department of Physiotherapy, University for the Development of the State and Pantanal Region (UNIDERP), Campo Grande – MS

Ana Paula da Costa Marques, UFMS

Professor of Universidade Federal de Mato Grosso do Sul – UFMS, MS

Geisa Poliane de Oliveira, Uniderp, MS

Physiotherapist, Universidade para o Desenvolvimento do Estado e da Regão do Pantanal – Uniderp, MS

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Publicado

11.03.2009

Como Citar

1.
de Carvalho P de TC, Reis FA dos, Belchior ACG, Silva BAK da, Pereira DM, Bento NC, et al. In vivo killing of Staphylococcus aureus by toluidine blue-mediated photosensitization in an animal model wounds. Cons. Saúde [Internet]. 11º de março de 2009 [citado 17º de julho de 2024];7(4):423-30. Disponível em: https://periodicos.uninove.br/saude/article/view/1415

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