In vivo killing of Staphylococcus aureus by toluidine blue-mediated photosensitization in an animal model wounds

Paulo de Tarso Camillo de Carvalho, Filipe Abdalla dos Reis, Ana Carulina Guimarães Belchior, Baldomero Antônio Kato da Silva, Daniel Martins Pereira, Nadia Cabral Bento, Ana Paula da Costa Marques, Geisa Poliane de Oliveira

Resumo


The aims of this study were to determine whether S. aureus could be killed by toluidine blue-mediated photosensitization in vivo in an animal model. Twelve Wistar rats divided into three groups (n = 12): Group I: Control Group, the wounds were made and submitted to the application of the laser without the drug photosensitizing; Group II: The implementation of wounds received the toluidine blue, without application of laser; Group III: it was used toluidine blue, and application of laser-Indio phosphide Gallium-Aluminum (InGaAIP)  660nm power and density of 20 Joules/cm2. Statistical analysis of CFU by analysis of variance Kruskal-Wallis test shows significant intraoperative difference, photodynamic therapy group (p < 0, 05), the Dunns post hoc test shows significant difference between Group I when compared to Group II treated with LLLT (p < 0001). The results of this study show that photodynamic therapy with toluidine blue has reduced the number of Staphylococcus aureus in vivo.

Palavras-chave


Photodynamic therapy; Toluidine blue; Wound infection.

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DOI: https://doi.org/10.5585/conssaude.v7i4.1415

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