In vivo killing of Staphylococcus aureus by toluidine blue-mediated photosensitization in an animal model wounds

Resumo

The aims of this study were to determine whether S. aureus could be killed by toluidine blue-mediated photosensitization in vivo in an animal model. Twelve Wistar rats divided into three groups (n = 12): Group I: Control Group, the wounds were made and submitted to the application of the laser without the drug photosensitizing; Group II: The implementation of wounds received the toluidine blue, without application of laser; Group III: it was used toluidine blue, and application of laser-Indio phosphide Gallium-Aluminum (InGaAIP)  660nm power and density of 20 Joules/cm2. Statistical analysis of CFU by analysis of variance Kruskal-Wallis test shows significant intraoperative difference, photodynamic therapy group (p < 0, 05), the Dunns post hoc test shows significant difference between Group I when compared to Group II treated with LLLT (p < 0001). The results of this study show that photodynamic therapy with toluidine blue has reduced the number of Staphylococcus aureus in vivo.