Fotobiomodulação com LED induz melhora na funcionalidade da marcha em ratos após lesão nervosa periférica

Carla Bernardo Soldera; Valéria Araújo-Gregio; Lucas Andreo; Tainá Caroline dos Santos Malavazzi; Fernanda Rocha Silva; Alessandro Melo Deana; Kristianne Porta Santos Fernandes; Raquel Agnelli Mesquita-Ferrari

doi:10.5585/21.2022.23401

Authors

Carla Bernardo Soldera Universidade Nove de Julho (UNINOVE). https://orcid.org/0000-0003-4797-0250
Valéria Araújo-Gregio Universidade Nove de Julho (UNINOVE). https://orcid.org/0000-0003-3477-7232
Lucas Andreo Universidade Nove de Julho (UNINOVE). https://orcid.org/0000-0001-8313-4300
Tainá Caroline dos Santos Malavazzi Universidade Nove de Julho (UNINOVE). https://orcid.org/0000-0002-2770-2753
Fernanda Rocha Silva Universidade Nove de Julho (UNINOVE). https://orcid.org/0000-0001-5584-6116
Alessandro Melo Deana Universidade Nove de Julho (UNINOVE). https://orcid.org/0000-0002-0014-6953
Kristianne Porta Santos Fernandes Universidade Nove de Julho (UNINOVE). https://orcid.org/0000-0001-7156-9286
Raquel Agnelli Mesquita-Ferrari Universidade Nove de Julho (UNINOVE). https://orcid.org/0000-0001-5142-9526

DOI:

https://doi.org/10.5585/21.2022.23401

Keywords:

Peripheral nerve injury, Photobiomodulation, LED, Nerve regeneration, Sciatic nerve.

Abstract

Introduction: Peripheral nerve injuries (PNI) can result in motor and sensory disturbances altering the functionality of the affected limb, however not much is known about the effects of photobiomodulation (PBM) with light emitting diode (LED). Objective: We aimed to analyze the effects of LED on the gait function of Wistar rats after PNI.
Methodology: Wistar rats were submitted to PNI by sciatic crush and analyzed in the following experimental groups: (1) Control; (2) PNI; (3) PNI+ LED (780 nm, mean power 40 mW, radiant exposure, energy per spot, 3.2 J on the sciatic nerve) (LEDn); (4) LNP+ LED on nerve and involved muscle region (LEDnm) and (5) LNP+ LED only on muscle region (LEDm). After 7-, 14-, 21- and 28-days gait analyses were performed using the Sciatic Functional Index (SFI).
Results: After 7 days, the groups treated with LED showed an improvement in gait compared to the PNI group, with this improvement being more pronounced in the LEDn group. After 14 days, the LEDn and LEDnm groups showed similar values to the control group and after 21 and 28 days the SFI did not show differences between the experimental groups.
Conclusion: LED increased the gait functionality evaluated by SFI after 1 and 2 weeks post-PNI, especially when it was used in the nerve region associated or not with the muscle region.

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Author Biography

Raquel Agnelli Mesquita-Ferrari, Universidade Nove de Julho (UNINOVE).

Atua principalmente nas áreas de plasticidade muscular (in vivo e in vitro), biologia molecular, inflamação e reparo tecidual, fotobiomodulação e fisioterapia aplicada a reumatologia, dermato-funcional e ginecologia e obstetrícia. Atualmente é professora dos Programa de Mestrado e Doutorado em Ciências da Reabilitação e Mestrado e Doutorado em Biofotônica aplicada às Ciências da Saúde além de atuar como docente em cursos de especialização em Saúde e da graduação em Fisioterapia e Medicina da Universidade Nove de Julho (UNINOVE).

References

Modrak M, Talukder MAH, Gurgenashvili K, Noble M, Elfar JC. Peripheral nerve injury and myelination: Potential therapeutic strategies. J Neurosci Res. 2020 May 13;98(5):780–95.

Taylor CA, Braza D, Rice JB, Dillingham T. The incidence of peripheral nerve injury in extremity trauma. American J Phys Med Rehab. 2008 May;87(5):381–5.

Rochkind S, Geuna S, Shainberg A. Phototherapy and nerve injury: Focus on muscle response [Internet]. 1st ed. Vol. 109, International Review of Neurobiology. Elsevier Inc.; 2013. 99–109 p. Available from: http://dx.doi.org/10.1016/B978-0-12-420045-6.00004-3

Andreo L, Soldera CB, Ribeiro BG, de Matos PRV, Bussadori SK, Fernandes KPS, et al. Effects of photobiomodulation on experimental models of peripheral nerve injury. Vol. 32, Lasers in Medical Science. Springer London; 2017. p. 2155–65.

Andreo L, Ribeiro BG, Alves AN, Martinelli ASA, Soldera CB, Horliana ACRT, et al. Effects of Photobiomodulation with Low‐level Laser Therapy on Muscle Repair Following a Peripheral Nerve Injury in Wistar Rats. Photochem Photobiol. 2020 Sep 23;96(5):1124–32.

Martignago CCS, Tim CR, Assis L, da Silva VR, Santos ECB dos, Vieira FN, et al. Effects of red and near-infrared LED light therapy on full-thickness skin graft in rats. Lasers Med Sci. 2020 Feb 5;35(1):157–64.

Hu D, Zhu S, Potas JR. Red LED photobiomodulation reduces pain hypersensitivity and improves sensorimotor function following mild T10 hemicontusion spinal cord injury. J Neuroinflammation. 2016 Dec 26;13(1):200.

Takhtfooladi MA, Shahzamani M, Takhtfooladi HA, Moayer F, Allahverdi A. Effects of light-emitting diode (LED) therapy on skeletal muscle ischemia reperfusion in rats. Lasers Med Sci. 2015 Jan 2;30(1):311–6.

de Melo CAV, Alves AN, Terena SML, Fernandes KPS, Nunes FD, da Silva D de FT, et al. Light-emitting diode therapy increases collagen deposition during the repair process of skeletal muscle. Lasers Med Sci. 2016;31(3):531–8.

Araujo T, Andreo L, Tobelem D da C, Silva T, Malavazzi TC dos S, Martinelli A, et al. Effects of systemic vascular photobiomodulation using LED or laser on sensory–motor recovery following a peripheral nerve injury in Wistar rats. Photochemical & Photobiological Sciences. 2022 Nov 9;

Bain. J. R., Mackinnon SE, Hunter DA. Functional evaluation of complete sciatic peroneal and posterior tibial nerve lesions in the rat. Plastic Reconstr Surg. 1989;83(1):129–36.

Klusáková I, Dubový P. Experimental models of peripheral neuropathic pain based on traumatic nerve injuries – An anatomical perspective. Annals of Anatomy - Anatomischer Anzeiger. 2009 Jan;191(3):248–59.

Kontinen VK, Meert TF. Vocalization Responses After Spinal Administration of Bicuculline or Strychnine in Rats. Neuromodulation: Technology at the Neural Interface. 2002 Oct;5(4):221–30.

Anders JJ, Geuna S, Rochkind S. Phototherapy promotes regeneration and functional recovery of injured peripheral nerve. Neurol Res. 2004 Mar 19;26(2):233–9.

Serafim KGG, de Paula Ramos S, de Lima FM, Carandina M, Ferrari O, Dias IFL, et al. Effects of 940 nm light-emitting diode (led) on sciatic nerve regeneration in rats. Lasers Med Sci. 2012 Jan;27(1):113–9.

Oliveira FB, Pereira VMD, Trindade APNT da, Shimano AC, Gabriel RECD, Borges APO. Ação do laser terapêutico e do ultrassom na regeneração nervosa periférica. Acta Ortop Bras. 2012;20(2):98–103.