Study histological and biomechanical of the effects of low-level Laser therapy, 660nm, on bone defects in tibia of rats

Abstract

Introduction: Consolidation of difficult fractures are commonly found and associated with high rates of morbidity. Within this context, the use of low level Laser therapy have been studied in an attempt to minimize the time of bone consolidation. Objective: To analyze the effects of low level Laser therapy, λ = 660 nm, with fluences of 60J/cm² and 120J/cm2, on bone defects in tibia of rats. Methods: We used 30 rats, distributed in the following groups: 1) control (with bone defect, without treatment); 2) bone defect, treated with Laser 660nm, 30mW, fluence 60J/cm2; 3) bone defect, treated with Laser 660nm, 30mW, fluence 120J/cm2. The animals were sacrificed 14 days after the defect, and the tibia followed for histological and biomechanical analysis. Results: There were no statistical diference in histological and biomechanical analysis. Conclusion: The Laser 660nm did not accelerate the healing process and did not improve the mechanical properties of bone callus.